In compliance with federal regulations the VDC will handle samples submitted for Brucellosis testing as follows:
- VDC is currently approved to run the buffered acidified plate antigen (BAPA), Card, Rivanol, and Standard Plate (SPT) Brucellosis serological tests.
- Non Export Testing - The BAPA will be used as the primary test for all species. Samples with non-negative results, except swine samples, will be submitted to the National Veterinary Services Laboratories (NVSL) for supplemental testing. Swine samples non-negative on the screening test will have additional testing done at VDC. If the supplemental test is still non-negative, they will be submitted to NVSL for additional testing.
- Card Test - Cattle, bison and cervid samples non-negative on the card test are classified as reactors pending supplemental testing. If supplemental testing is negative, the animal can be reclassified as a suspect, but not reclassified as negative. Therefore, the card test should be only be used in these species when absolutely necessary.
- Export - Samples for international export must be tested as specified by the receiving country. Non-negative samples are forwarded to NVSL for supplemental testing. International export requirements can be determined by calling the USDA Veterinary Services Office at 402-434-2300 and are also listed at the following website.
http://www.aphis.usda.gov/regulations/vs/iregs/animals/
Testing requires at least 2.0 ml of serum. Samples must be submitted to the Nebraska Veterinary Diagnostic Center along with an Official Brucellosis Test Record (VS From 4-33). Samples will generally be tested on the day of receipt, but submitting veterinarians are encouraged to contact the laboratory regarding testing needs for large numbers of samples (>100) or special time constraints.
Our fees for Brucellosis testing at the VDC are as follows:
$12.00 Accession fee
Card Test $2.20
Standard Plate Test - $4.00
BAPA Test - $2.20
Rivanol - $5.00
$1.10/sample for serum separation as required.
Test results will be communicated to the submitting veterinarian through the state of Nebraska Bureau of Animal Industry.
Official Brucellosis Test Record (VS From 4-33) forms may be ordered by calling the USDA APHIS Veterinary Services area office (402-434-2300).
Please contact Dr. J. Dustin Loy at the VDC (402) 472-1434 or the lab (402) 472-8470 with questions