Tritrichomonas foetus Testing

Tritrichomonas foetus testing

Submission guidelines

Cost and detailed information

Trich Direct Realtime PCR (Yellow cap tube with PBS media supplied by NVDC)
Trich Direct Pooled Realtime PCR (pool up to 5 samples) (Yellow cap tube with PBS media supplied by NVDC)
Trichomonas foetus Realtime PCR in TF media (Red cap TF tube or pouch)
Trichomonas foetus Realtime PCR in TF Media (pool up to 5 samples) (Red cap TF tube or pouch)
Tritrichomonas foetus culture (Pouch) Bovine only
Trich In-pouch PCR processing fee

Prices are current as of Oct 1, 2020. Subject to change. Accession fees/shipping or other charges not included.

Submission information

Collection and submission requirements for Tritrichomonas foetus (TF) testing using Direct-RT qPCR.

  • PBS Transport tubes (yellow caps) must be ordered from NVDC to ensure quality control and collection container consistency. Please contact the laboratory at 402-472-8470 to order tubes. These will be provided at no cost to clientele who would like to submit samples to the NVDC for the direct PCR test.
  • PBS transport tubes (yellow caps) have a 1-2 year shelf life. Tubes should be kept in a cool dark place or refrigerated to ensure quality and sterility.
  • Smegma from preputial scrapings or washes from bulls must be inoculated into PBS immediately after collection. Once inoculated, samples must either be refrigerated at 4°C and received within 5 days post-collection or frozen at -20°C, and received within 7 days post-collection. Samples should be mailed with ice packs, in an insulated container (i.e., Styrofoam cooler), that has been placed inside of another shipping container.
  • You can generate overnight shipping labels from our website  using the links in the "How to generate a UPS EZ-Ship label" section. It is recommended to select the next day air option (especially if you live outside the “one-day ground” zone; see map on shipping page).
  • Samples submitted in TF PBS transport tubes do not require incubation and will be processed upon arrival.
  • Pooling of samples can only be performed by the laboratory (up to 5 samples/pool). Samples cannot be pre-pooled within tubes by the submitting veterinarian. Please be sure to request pooling on the submission form if you would like pooled testing performed.
  • Please ensure direct PCR testing meets the requirements of the importing state if these tests are for interstate movement. The collection media will be indicated on the laboratory report.
  • NVDC will continue to accept samples in TF transport tubes (red caps) or TF Pouch samples that are in date.
  • TF transport tubes (red caps) do not require incubation and should either be refrigerated at 4°C and received by the laboratory within 5 days post-collection or frozen at -20°C and received by the laboratory within 14 days post-collection. After inoculation, samples should be shipped on ice.
  • In-Pouches must be incubated prior to testing. If incubation is performed prior to shipment to the laboratory, samples may be refrigerated at 4°C and received by the laboratory within 5 days post-collection or frozen at -20°C and received by the laboratory within 14 days post-collection. After inoculation and incubation, samples should be shipped on ice. If incubation cannot be performed prior to shipping, In-pouches must be kept in the dark, at room temperature, and received by the laboratory within 5 days post-collection.

Please call 402-472-8470 with any questions.