Tritrichomonas foetus testing
Cost and detailed information
Prices are current as of Oct 1, 2020. Subject to change. Accession fees/shipping or other charges not included.
Collection and submission requirements for Tritrichomonas foetus (TF) testing using Direct-RT qPCR.
- PBS Transport tubes (yellow caps) must be ordered from NVDC to ensure quality control and collection container consistency. Please contact the laboratory at 402-472-8470 to order tubes. These will be provided at no cost to clientele who would like to submit samples to the NVDC for the direct PCR test.
- PBS transport tubes (yellow caps) have a 1-2 year shelf life. Tubes should be kept in a cool dark place or refrigerated to ensure quality and sterility.
- Smegma from preputial scrapings or washes from bulls must be inoculated into PBS immediately after collection. Once inoculated, samples must either be refrigerated at 4°C and received within 5 days post-collection or frozen at -20°C, and received within 7 days post-collection. Samples should be mailed with ice packs, in an insulated container (i.e., Styrofoam cooler), that has been placed inside of another shipping container.
- You can generate overnight shipping labels from our website using the links in the "How to generate a UPS EZ-Ship label" section. It is recommended to select the next day air option (especially if you live outside the “one-day ground” zone; see map on shipping page).
- Samples submitted in TF PBS transport tubes do not require incubation and will be processed upon arrival.
- Pooling of samples can only be performed by the laboratory (up to 5 samples/pool). Samples cannot be pre-pooled within tubes by the submitting veterinarian. Please be sure to request pooling on the submission form if you would like pooled testing performed.
- Please ensure direct PCR testing meets the requirements of the importing state if these tests are for interstate movement. The collection media will be indicated on the laboratory report.
- NVDC will continue to accept samples in TF transport tubes (red caps) or TF Pouch samples that are in date.
- TF transport tubes (red caps) do not require incubation and should either be refrigerated at 4°C and received by the laboratory within 5 days post-collection or frozen at -20°C and received by the laboratory within 14 days post-collection. After inoculation, samples should be shipped on ice.
- In-Pouches must be incubated prior to testing. If incubation is performed prior to shipment to the laboratory, samples may be refrigerated at 4°C and received by the laboratory within 5 days post-collection or frozen at -20°C and received by the laboratory within 14 days post-collection. After inoculation and incubation, samples should be shipped on ice. If incubation cannot be performed prior to shipping, In-pouches must be kept in the dark, at room temperature, and received by the laboratory within 5 days post-collection.
Please call 402-472-8470 with any questions.