Matthew Wiebe

Dr. Matthew Wiebe

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Faculty Profile


My research focuses on understanding interactions between human DNA viruses and defensive mechanisms employed by their hosts to target viral genomes. My laboratory utilizes both classical molecular virology techniques and state-of-the-art genomics and proteomic approaches in our work. Current projects include efforts to characterize how evolutionarily connected signaling pathways present in viruses and their hosts converge and compete for control of host antiviral effectors.


  • Postdoctoral in Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI, 2009
  • P.H.D. in Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, 2003
  • B.S. in Biotechnology, University of Nebraska at Omaha, Omaha, NE, 1994


Rico AB, Linville AC, Olson AT, Wang Z, Wiebe MS. The VACV B12 Pseudokinase Represses Viral Replication via Interaction with the Cellular Kinase VRK1 and Activation of the Antiviral Effector BAF. J Virol. 2021 Jan 13;.doi: 10.1128/JVI.02114-20. PubMed PMID: 33177193. 

Rico AB, Wang Z, Olson AT, Linville AC, Bullard BL, Weaver EA, Jones C, Wiebe MS. The Vaccinia B1 and Cellular VRK2 Kinases Promote Vaccinia Replication Factory Formation through Phosphorylation Dependent Inhibition of Vaccinia B12. Journal of Virology 2019 Jul 24;. doi: 10.1128/JVI.0085519.  PubMed PMID: 31341052.   

Olson AT, Wang Z, Rico AB, Wiebe MS. A poxvirus pseudokinase represses viral DNA replication via a pathway antagonized by its paralog kinase. PLOS Pathogens. Feb 15 2019. PMID: 30768651.   

Rico AB, Olson AO, Wiebe MS. Generation of Vaccinia Virus Gene Deletion Mutants Using Complementing Cell Lines. Methods in Molecular Biology: Vaccinia Virus- Methods and Protocols. 2019. 

Olson AT, Rico AB, Wang Z, Delhon G, Wiebe MS. Deletion of the Vaccinia B1 Kinase Reveals Essential Functions of this Enzyme Complemented Partly by the Homologous Cellular Kinase VRK2. J Virology. 2017 Jul 12;91(15).  PMID: 28515294.   

Wiebe MS, Jamin A.  The Barrier to Autointegration Factor: Interlocking Antiviral Defense with Genome Maintenance.  J Virol. 2016 Mar 28;90(8):3806-9. Review.  PMID: 26842478 

Jamin A, Ibrahim N, Wicklund A, Weskamp K, Wiebe MS. Vaccinia B1 kinase is required for postreplicative stages of the viral lifecycle in a BAF-independent manner in U2OS cells. J Virology. 2015 Jul 29. PubMed PMID: 26223647   

Jamin A, Wiebe MS. Barrier to Autointegration Factor (BANF1): interwoven roles in nuclear structure, genome integrity, innate immunity, stress responses and progeria. Curr Opin Cell Biol. 2015 Jun 10;34:6168. Review. PubMed PMID: 26072104. 

Jamin A, Thunuguntla P, Wicklund A, Jones C, Wiebe MS. Barrier to Autointegration Factor Becomes Dephosphorylated During HSV-1 Infection and Can Act as a Host Defense by Impairing Viral DNA Replication and Gene Expression. PLoS ONE 2014 Jun 19;9(6):e100511. 

Jamin A, Wicklund A, Wiebe MS. Cell and Virus Mediated Regulation of the Barrier-to-Autointegration Factor's Phosphorylation State Controls its DNA Binding, Dimerization, Subcellular Localization, and Antipoxviral Activity. J Virol. 2014 Mar 5;PubMed PMID: 24600006.   

View Complete List of Published Work in My Bibliography


  • NIH R56 AI153099
    Wiebe (P.I.)
    Engagement of Cellular Mitotic and Antiviral Signaling by Poxviral Kinases
    The goal of this project is to determine how viral kinases repurpose cellular signaling pathways and impact the outcome of poxvirus infection.
  • NIH R01 AI114653-01A1
    Wiebe (P.I.)
    Mechanism of the Antiviral Activity of BAF Against Poxvirus and HSV-1 Infection
    The goal of this project is to determine how cellular effectors detect and impair foreign DNA, and how these mechanisms are inactivated by viruses.


MOLR 139
Nebraska Center for Virology
4240 Fair Street
Lincoln, NE 68583-0900


75% Research, 25% Teaching

Research Areas

  • Molecular and Cellular Biology
  • Virology

Professional Society Memberships

  • American Association for the Advancement of Science
  • American Society for Virology
  • American Society for Microbiology