You (Joe) Zhou

Dr. You (Joe) Zhou
Research Professor

We believe in the power of every person.

Faculty Profile


  • Postdoctoral Fellow in Molecular Cell Biology/Microscopy, University of Toronto, Toronto, Ontario, Canada
  • Ph.D. in Animal Cell Biology, Department of Zoology, University of Western Ontario, London, Ontario
  • B.S. in Animal Histology/Cell Biology, Nanchang University (former Jiangxi University), Nanchang, Jiangxi, R.R. China

Professional Responsibilities and Activities

  • Director, Microscopy Research Core Facility
    Center for Biotechnology, University of Nebraska-Lincoln, NE
  • Courtesy Faculty, School of Biological Sciences
    University of Nebraska-Lincoln, NE
  • Research Professor, School of Veterinary Medicine & Biomedical Sciences
    University of Nebraska-Lincoln, NE
  • Research Associate Professor, Department of Veterinary & Biomedical Sciences
    University of Nebraska-Lincoln, NE
  • Research Assistant Professor, School of Biological Sciences
    Department of Veterinary & Biomedical Sciences
    University of Nebraska-Lincoln, NE
  • Director, Antibody Core Research Facility
    Center for Biotechnology
    University of Nebraska-Lincoln, NE
  • Instructor-Research Faculty, Department of Neurosciences
    Pennington Biomedical Research Center
    Louisiana State University, Baton Rouge, LA


For more up-to-date listings of publications, visit the following:

  1. Zhou, Y. 1983. Blood micro-circulation pathways in human: structure and function. Jiangxi J. Chinese Med 83(3):29-32.
  2. Zhou, Y. 1984. The collateral circulation. Biol. Bull. (Chinese) 84(5):32-34.
  3. Dai, H. and Y. Zhou. 1987. A histochemical DAB method for visualization of micro-blood vessels. Chinese J. Anat. 10(3):246-247.
  4. Zhou, Y. 1988. An overview of biomembrane structure. Biol. Bull. (Chinese) 88(1):9-11.
  5. Dai, H., X. Zeng, Y. Zhou, H. Wang, and X. Chang. 1989. Experimental studies on the renal vessel ligation in rabbits. Acta Anat. Sinica (JEIPOU XUEBAO) 20(2):121-124.
  6. Zhou, Y. and R.B. Podesta. 1989. Surface spines of human blood flukes (Schistosoma mansoni) contain bundles of actin filaments having identical polarity. Eur. J. Cell Biol. 48:150-153.
  7. Zhou, Y. and R.B. Podesta. 1989. Effects of serotonin (5HT) and complement C3 on the synthesis of the surface membrane precursors of adult Schistosoma mansoni. J. Parasitol 75: 333-343.
  8. Siddiqui, A.A., Y. Zhou, R.B. Podesta, and M.W. Clarke. 1990. Isolation of a highly enriched plasma membrane fraction of Trypanosoma brucei by free-flow electrophoresis. Mol Biochem Parasitol 40(1):95-104.
  9. Zhou, Y., S.R. Karcz, and R.B. Podesta. 1990. Localization of the 24 kilodalton glycoprotein in adult Schistosoma mansoni using immunofluorescence and immunoelectron microscopy. J Parasitol 76(4):601-605.
  10. Zhou, Y. and R.B. Podesta. 1992. Ring-shaped organization of cytoskeletal F-actin associated with surface sensory receptors of the human blood fluke, Schistosoma mansoni: A confocal and electron microscopic study. Tissue & Cell. 24(1):37-49.
  11. Siddiqui, A.A., Y. Zhou, R.B. Podesta, S.K. Karcz, G.H. Strejan, G.A. Dekaban, and M.W. Clarke. 1993. Characterization of Ca++-dependent neutral protease (calpain I & II) from human blood flukes, Schistosoma mansoni. Biochem Biophys Acta 1181:37-44.
  12. Zhou, Y., E. Dziak, and M. Opas. 1993. Adhesiveness and proliferation of epithelial cells are differentially modulated by activation and inhibition of protein kinase C in a substratum-dependent manner. J Cell Physiol 155:14-26.
  13. Zhou, Y., Anna Moszczynska, and M. Opas. 1994. Generation and characterization of specific antibodies to adhesion-related molecules of RPE cells. Exp Eye Res 58: 585-594.
  14. Zhou, Y., E. Dziak, T.R. Unnasch, and M. Opas. 1994. Major retinal cell components recognized by onchocerciasis sera are associated with the cell surface and nucleoli. Invest. Ophthalmol. Visual Sci 35:1089-1099.
  15. Zhou, Y. and M. Opas. 1994. The cell shape, intracellular pH, N-CAM expression and FGF responsiveness during transdifferentiation of retinal pigment epithelium into neuroepithelium in vitro. Biochem Cell Biol 72:257-265
  16. Zhou, Y., E.M. Macus, R.P. Haugland, and M. Opas. 1995. Use of a new fluorescent probe, SNAFL-calcein, for determination of intracellular pH by simultaneous dual-emission imaging laser scanning confocal microscopy. J Cell Physiol 164:9-16.
  17. Snow, B.E., H.H.Q. Heng, X.M. Shi, Y. Zhou, K. Du, R. Taub, L.-C. Tsui, and R.R. McInnes. 1997. Expression Analysis and Chromosomal Assignment of the human SRp40 gene. Genomics 43:165-170.
  18. Rybkin, I.I., Y. Zhou, J. Volaufov, G.N. Smagin, D.H. Ryan, and R.B.S. Harris. 1997. The effects of restraint stress on food intake and body weight is determined by time of day. Am. J. Physiol. 273 (42): R1612-R1622.
  19. Zhou, Y., P.K. Elkins, L.A. Howell, D.H. Ryan, and R.B.S. Harris. 1998. Apolipoprotein-E deficiency results in altered stress responsiveness in addition to an impaired spatial memory in young mice. Brain Res. 788(1-2): 151-160.
  20. Zhou, Y., A. Cheshire, L.A. Howell, D.H. Ryan, and R.B.S. Harris. 1999. Neuro-autoantibody reactivity in relation to aging and stress in apolipoprotein E-deficient mice. Brain Res. Bul., 49:173-179.
  21. Opas M, J.R. Davies, Y. Zhou, E. Dziak. 2001. Formation of retinal pigment epithelium in vitro by transdifferentiation of neural retina cells. Int. J. Dev. Biol. 45(4):633-42.
  22. Korotkov, K.V., E. Kumaraswamy, Y. Zhou, D.L. Hatfield and V. N. Gladyshev. 2001. Association between the 15 kDa selenoprotein and UDP glucose:glycoprotein glucosyltransferase in the endoplasmic reticulum of mammalian cells. J. Biol. Chem. 276:15330-15336.
  23. Li, L.J., K. Arumuganathan, H.W. Rines, R.L. Phillips, O. Riera-Lizarazu, D. Sandhu, Y. Zhou, K.S. Gill,. 2001. Flow Cytometric sorting of maize chromosome 9 from an oat-maize addition line. Theoretical Applied Genetics 102:658-663.
  24. Ross, E.J.H, L. Shearman, M. Mathiesen, Y. Zhou, R. Arredondo-Peter, G. Sarath, R. V. Klucas 2001. Non-symbiotic hemoglobins in rice are expressed during germination and in differentiating cell types. Protoplasma 218:125-133.
  25. Datta R, P. Choudhury, M. Bhattacharya M, F. Soto Leon, Y. Zhou, B. Datta. 2001. Protection of translation initiation factor eIF2 phosphorylation correlates with eIF2-associated glycoprotein p67 levels and requires the lysine-rich domain I of p67. Biochimie 83(10):919-931.
  26. Chowdhury, S.I., P.S. Bhattachajee, A. Al-Mubarak, M.L. Weiss, M. Onderci, and Y. Zhou. 2002. Bovine Herpesvirus 5 (BHV-5) Us9 Is Essential for BHV-5 Neuropathogenesis. J. Virol. 76 (8):3839-51.
  27. Buhr, T., S. Sato, F. Ebrahim, A. Xing, Y. Zhou, M. Mathiesen, B. Sweiger, A. Kinney, P. Staswick, and T. Clemente. 2002. Ribozyme termination of RNA transcripts down-regulate seed fatty acid genes in transgenic soybean. Plant J. 30(2):155-163. 
  28. Komina, O., Y. Zhou, G. Sarath, R. Chollet. 2002. In Vivo and In Vitro Phosphorylation of Membrane and Soluble Forms of Soybean Nodule Sucrose Synthase (Nodulin-100). Plant Physiol. 129(4):1664-1673.
  29. Vaidyanathan, H., Y. Zhou, T. M. Petro and S.D. Schwartzbach. 2003. Intracellular localization of the p35 subunit of murine IL-12. Cytokine Feb 7; 21(3):120-128.
  30. Dasgupta S., Y. Zhou, M. Jana, N.L. Banik and K. Pahan. 2003. Sodium phenylacetate inhibits the adoptive transfer of experimental allergic encephalomyelitis in SJL/J mice at multiple steps. J. Immunol. 170(7):3874-82.
  31. Ryan, L., Peng, H., Erichsen, D., Huang, Y., Persidsky, Y. Zhou, H.E. Gendelman, and J. Zheng. 2004. Links between Tumor Necrosis Factor Related Apoptosis Inducing Ligand Mediated Human Neuronal Apoptosis and HIV-1 Associated Dementia. J. Neuroimmunol. 148: 127-139.
  32. Xu, W., Y. Zhou, R. Chollet. 2003. Identification and expression of a soybean nodule-enhanced PEP-carboxylase kinase gene (NE-PpcK) that shows striking up-/down-regulation in vivo. Plant J. 34(4):441-52.
  33. Chen, K. C-W. Y. Zhou, K. Xing, K. Krysan and M.F. Lou 2004. Platelet Derived Growth Factor (PDGF)-induced Reactive Oxygen Species in the Lens Epithelial Cells: The Redox Signaling. Exp. Eye Res. 78:1057-1067.
  34. Al-Mubarak, A., Y. Zhou, S.I. Chowdhury 2004. A glycine-rich BHV-5 gE-specific epitope within the ectodomain is important for BHV-5 neurovirulence. J Virol. 78:4806-16
  35. Berberov, E.M. Y. Zhou, D. H. Francis, M.A. Scott, S.D. Kachman, and R.A. Moxley 2004. Relative importance of heat-labile enterotoxin in the causation of severe diarrheal disease in the gnotobiotic piglet model by a strain of enterotoxigenic Escherichia coli that produces multiple enterotoxins. Infect Immun. 72(7):3914-24.
  36. Dasgupta S, M. Jana M, Y. Zhou, Y.K. Fung, S. Ghosh, K. Pahan. 2004 Antineuro-inflammatory effect of NF-kappa-B essential modifier-binding domain peptides in the adoptive transfer model of experimental allergic encephalomyelitis. J. Immunol. 173(2):1344-54.
  37. Inman M, J(Y). Zhou, H. Webb, C. Jones. 2004. Identification of a novel bovine herpesvirus 1 transcript containing a small open reading frame that is expressed in trigeminal ganglia of latently infected cattle. J. Virol. 78 (10):5438-47.
  38. Vitvitsky V, S. Dayal, S. Stabler, Y. Zhou, H. Wang, S.R. Lentz, R. Banerjee. 2004. Perturbations in homocysteine-linked redox homeostasis in a murine model for hyperhomocysteinemia. Am J Physiol. Regul. Integr. Comp. Physiol. 287(1):R39-46. 
  39. Zhang L, Zhang J, Lambert Q, Der CJ, Del Valle L, Miklossy J, Khalili K, Zhou Y, Pagano JS. 2004 Interferon regulatory factor 7 is associated with Epstein-Barr virus-transformed central nervous system lymphoma and has oncogenic properties. J Virol. 78(23):12987-95.
  40. Liu, Y., Z. Liu, H. Gao, Y. Zhou, E. J. Androphy and J. J. Chen. 2005 Opposing Effects of Bovine Papillomavirus Type 1 E6 and E7 Genes on Fas-Mediated Apoptosis. Oncogene 24(24):3942-53
  41. Richard, D.S., R. Rybczynski, T.G. Wilson, Y. Wang, M.L. Wayne, Y. Zhou, L. Partridge and L.G. Harshman. 2005. Insulin signaling is necessary for vitellogenesis in Drosophila melanogaster independent of the roles of juvenile hormone and ecdysteroids: female sterility of the chico insulin signaling mutation is autonomous to the ovary. J. Insect Physiol. 51: 455-464.
  42. Rhoads, D. M., S. J. White, Y. Zhou, M. Muralidharan, T. E. Elthon. 2005. Altered gene expression in plants with constitutive expression of mitochondrial small heat shock protein suggests the involvement of retrograde regulation in the heat stress response. Physiol. Plantarum 123:435–444.
  43. Dassanayake, R.P., Y. Zhou, S. Hinkley, C.J. Stryker, G. Plauche, J.T. Borda, K. Sestak, and G.E. Duhamel. 2005. Characterization of cytolethal distending toxin of campylobacter species isolated from captive macaque monkeys. J Clin Microbiol. 43(2):641-9.
  44. Zhang Y, Zhou J(Y), Jones C. 2005 Identification of functional domains within the bICP0 protein encoded by bovine herpesvirus 1. J Gen Virol. 86:879-86.
  45. Su D, Novoselov SV, Sun QA, Moustafa ME, Zhou Y, Oko R, Hatfield DL, Gladyshev VN. 2005. Mammalian selenoprotein thioredoxin-glutathione reductase. Roles in disulfide bond formation and sperm maturation. J. Biol Chem. 280(28):26491-8. 
  46. Boehm, M.B., T.J. Milius, Y. Zhou, J. J. Westendorf, S. Koka, 2005. The mammalian formin FHOD1 interacts with the ERK MAP kinase pathway. Biochem. Biophys. Res. Commun. 335(4):1090-4.
  47. Koka S, Minick GT, Zhou Y, Westendorf JJ, Boehm MB. 2005. Src regulates the activity of the mammalian formin protein FHOD1. Biochem Biophys Res Commun. 336(4): 1285-91.
  48. Chowdhury, S.I. Al-Mubarak, A., Y. Zhou. 2006. The Us9 gene of bovine herpesvirus 1 (BHV-1) effectively complements a Us9-null strain of BHV-5 for anterograde transport, neurovirulence, and neuroinvasiveness in a rabbit model. J. Virol. 80:4396-405.
  49. Zhang, Y, Jiang Y, Geiser V, Zhou J(Y), Jones C. 2006. Bovine herpesvirus 1 immediate-early protein (bICP0) interacts with the histone acetyltransferase p300, which stimulates productive infection and gC promoter activity. J. Gen. Virol. 87:1843-51. 
  50. Das, S.C., D. Nayak, Y. Zhou, and A. K. Pattnaik. 2006. Visualization of intracellular transport of vesicular stomatitis virus nucleocapsids in living cells. J. Virol. 80: 6368-6377.
  51. Perez S, Lovato L, Zhou J(Y), Doster A, Jones C. 2006. Comparison of inflammatory infiltrates in trigeminal ganglia of cattle infected with wild-type Bovine herpesvirus 1 versus a virus strain containing a mutation in the LR (latency-related) gene. J Neurovirol. 2006 Oct;12(5):392-7.
  52. LaRosa, P.C., J. Miner, Y. Xia, Y. Zhou, S. Kachman and M. E. Fromm. 2006 Trans-10, cis-12 conjugated linoleic acid causes inflammation and delipidation of white adipose tissue in mice: a microarray and histological analysis. Physiol. Genomics Nov 27; 27(3):282-94.
  53. Kabil, O., Y. Zhou and R. Banerjee. 2006. Human cystathionine β-synthase is a target for sumoylation. Biochem. Nov 14;45(45):13528-36
  54. Xu, D. A. Buehner, J. Xu, T. Lambert, C. Nekl, M.K. Nielsen, Y. Zhou. 2006. A polymorphic glucocorticoid receptor in a mouse population may explain inherited altered stress response and increased anxiety-type behaviors. FASEB J Nov 20(13): E1802–E1810.
  55. Dou, H., J. Morehead, C. J. Destache, J. Limoges, B. Ellison, J. D. Kingsley, L. Shlyakhtenko, Y. Zhou, M. Chaubal, J. Werling, J. Kipp, B. Rabinow, and H. E. Gendelman. 2007. Laboratory investigations for the morphologic, pharmacokinetic, and anti-retroviral properties of indinavir nanoparticles in human monocyte-derived macrophages. Virol. 358(1):148-58.
  56. Saira K, Y. Zhou, C. Jones 2007. The infected cell protein 0 encoded by bovine herpesvirus 1 (bICP0) induces degradation of interferon response factor 3 (IRF3), and consequently inhibits beta interferon promoter activity. J. Virol. 81(7):3077-86. 
  57. Chen, K.C, Y. Zhou, W. Zhang, M.F. Lou. 2007. Control of PDGF-induced reactive oxygen species (ROS) generation and signal transduction in human lens epithelial cells. Mol. Vis. 13:374-87.
  58. Meyer F, Perez S, Jiang Y, Zhou Y, Henderson G, Jones C. 2007. Identification of a novel protein encoded by the latency-related gene of bovine herpesvirus 1. J Neurovirol. 2007 Dec;13(6):569-78
  59. LaRosa, P.C., J. M. Riethoven, H. Chen, Y. Xia, Y. Zhou, M. Chen, J. L. Miner, and M. E. Fromm. 2007. Trans-10, Cis-12 Conjugated Linoleic Acid Activates the Integrated Stress Response Pathway in Adipocytes. Physiol. Genomics Nov 14; 31(3):544-53.
  60. Zhang, Y., Y. Zhou, U. Schweizer, N. E. Savaskan, D. Hua, J. Kipnis, D. L. Hatfield, V. N. Gladyshev 2008. Comparative analysis of selenocysteine machinery and selenoprotein gene expression in mouse brain identifies key brain areas dependent on selenium. J. Biol. Chem.283: 2427-38.
  61. Zhou, Y., S. Zheng, W. Sprout, J. McDonald, M.K. Nielsen 2008. Additional evidence showing an additive effect of glucocorticoid receptor polymorphisms on anxiety-type behavior, stress response and body weight in a population of mice with low heat loss background. FASEB J. 22:6-8.
  62. Erume J, Berberov EM, Kachman SD, Scott MA, Zhou Y, Francis DH, Moxley RA. 2008. Comparison of the Contributions of Heat-Labile Enterotoxin and Heat-Stable Enterotoxin b to the Virulence of Enterotoxigenic Escherichia coli in F4ac Receptor-Positive Young Pigs. Infect Immun. 2008 Jul; 76(7):3141-9.
  63. Peng H, Whitney N, Wu Y, Tian C, Dou H, Zhou Y, Zheng J. 2008. HIV-1-infected and/or immune-activated macrophage-secreted TNF-alpha affects human fetal cortical neural progenitor cell proliferation and differentiation. Glia. 2008 Jun; 56(8):903-16.
  64. Saira K, Zhou Y, Jones C. 2009. The infected cell protein 0 encoded by bovine herpesvirus 1 (bICP0) associates with interferon regulatory factor 7 (IRF7), and consequently inhibits beta interferon promoter activity. J Virol. 2009 Apr; 83 (8):3977-81. 
  65. Kehr S, Malinouski M, Finney L, Vogt S, Labunskyy VM, Kasaikina MV, Carlson BA, Zhou Y, Hatfield DL, Gladyshev VN. 2009. X-Ray Fluorescence Microscopy Reveals the Role of Selenium in Spermatogenesis. J Mol Biol. 2009 Jun 26; 389 (5):808-18. 
  66. Wen HJ, Yang Z, Zhou Y, Wood C. 2010 Enhancement of autophagy during lytic replication by the Kaposi's sarcoma-associated herpesvirus replication and transcription activator. J Virol. 2010 Aug; 84(15):7448-58.
  67. Malinouski, M., Y. Zhou, V.V. Belousov, D.L. Hatfield, V.N. Gladyshev, 2011. Hydrogen peroxide probes directed to different cellular compartments. PLoS One. 2011 Jan 21; 6(1):e14564.
  68. Chen, Z.G., Z.P. Xu, M. Zhang, Y. Zhou, M. Liu, T. Patten, G.Y. Liu, H. Li, X.C. Zeng, L. Tan, 2012. "Two-Dimensional Crystallization of Hexagonal Bilayer with Moiré Patterns." J. Phys. Chem. B: 2012 Apr 12; 116(14):4363-9. Epub 2012 Mar 14 (PMID:22356348)
  69. Kanmogne, GD, S. Singh, U. Roy, X. Liu, J. McMillan, S. Gorantla, S. Balkundi, N. Smith, Y. Alnouti, N. Gautam, Y. Zhou, L. Poluektova, A. Kabanov, T. Bronich, and HE. Gendelman, 2012. Mononuclear phagocyte intercellular crosstalk facilitates transmission of cell-targeted nanoformulated antiretroviral drugs to human brain endothelial cells. Internat. J. Nanomed. May 2012 Vol 2012 (7): 2373-88
  70. Wu Y, Chen Q, Peng H, Dou H, Zhou Y, Huang Y, Zheng JC. 2012. Directed migration of human neural progenitor cells to interleukin-1β is promoted by chemokines stromal cell-derived factor-1 and monocyte chemotactic factor-1 in mouse brains. Transl Neurodegener. 2012 Jul 31; 1(1):15.
  71. Valente RM, Ehlers E, Xu D, Ahmad H, Steadman A, Blasnitz L, Zhou Y, Kastanek L, Meng B, Zhang L. 2012 Toll-like receptor 7 stimulates the expression of epstein-barr virus latent membrane protein 1. PLoS One. 2012; 7(8): e43317.
  72. Shibata A, McMullen E, Pham A, Belshan M, Sanford B, Zhou Y, Goede M, Date AA, Destache C. 2013. Polymeric nanoparticles containing combination antiretroviral drugs for HIV-1 treatment. AIDS Res Hum Retroviruses. 2013 May; 29(5):746-54. 
  73. Rodriguez-Rocha H, Garcia-Garcia A, Pickett C, Sumin L, Jones J, Chen H, Webb B, Choi J, Zhou Y, Zimmerman MC, Franco R. 2013. Compartmentalized oxidative stress in dopaminergic cell death induced by pesticides and complex I inhibitors: Distinct roles of superoxide anion and superoxide dismutases. Free Radic Biol Med. 2013 Apr 19. pii: S0891-5849(13)00161-5. 2013.04.021.
  74. Wang G, Xu Z, Chen Z, Niu W, Zhou Y, Guo J, Tan L. 2013. Sequential binding of large molecules to hairy MOFs. Chem Commun (Camb). 2013 Jun 27; 49(59):6641-3.
  75. Garcia-Garcia A1, Anandhan A, Burns M, Chen H, Zhou Y, Franco R. Impairment of Atg5-dependent autophagic flux promotes paraquat- and MPP⁺-induced apoptosis but not rotenone or 6-hydroxydopamine toxicity. Toxicol Sci. 2013 Nov;136(1):166-82.
  76. Frese SA, Mackenzie DA, Peterson DA, Schmaltz R, Fangman T, Zhou Y, Zhang C, Benson AK, Cody LA, Mulholland F, Juge N, Walter J. 2013 Molecular Characterization of Host-Specific Biofilm Formation in a Vertebrate Gut Symbiont. PLoS Genet. 2013 Dec 9(12): e1004057 (1-13).
  77. Massilamany, C., Gangaplara A, Jia T, Elowsky C, Kang G, Li Q, Zhou Y, and Reddy J. 2014. Direct staining with major histocompatibility complex class II dextramers permits detection of antigen-specific, autoreactive CD4 T cells in situ. PLoS One. 2014 Jan 27; 9(1):e87519. doi: 10.1371/journal.pone.0087519.
  78. Wang, L., G. Kang, P. Kumar, W. Lu, Y. Li, Y. Zhou, Q. Li and C. Wood. 2014. Humanized-BLT Mouse Model of Kaposi's Sarcoma-Associated Herpesvirus Infection. Proc Natl Acad Sci U S A. 2014 Feb 25; 111(8):3146-51. PMID: 24516154 
  79. Massilamany, C., Gangaplara A, Jia T, Elowsky C, Li Q, Zhou Y, and Reddy J. 2014. In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers: J. Vis. Exp. 2014 Aug 1; (90). doi: 10.3791/51679.
  80. Sanford B, Li Y, Maly CJ, Madson CJ, Chen H, Zhou Y, Belshan M. 2014. Deletions in the fifth alpha helix of HIV-1 matrix block virus release. Virol. 2014 Sep 10; 468-470C:293-302. PMID: 25217711
  81. Hu, M., T. Zhang, J. Stansbury, Y. Zhou, H. Chen, J. Neal. 2014. Contributions of Internal and External Fouling to Trans-membrane Pressure in MBRs: Experiments and Modeling. J. Environ. Eng. 2014 DOI: 10.1061/(ASCE) EE.1943-7870.0000925.
  82. Date, A.A., A. Shibata, E. McMullen, K. L. Bruzzo, P. Bruck, M. Belshan, Y. Zhou, C. J. Destache. 2015. Thermosensitive gel containing cellulose acetate phthalate-efavirenz combination nanoparticles for prevention of HIV-1 infection. J. Biomed. Nanotechnol. 11 (3), March 2015: 416-427.
  83. Mandal, S. Y Zhou, A. Shibata, and C. J. Destache. 2015 Confocal fluorescence microscopy: An ultra-sensitive tool used to evaluate intracellular antiretroviral nano-drug delivery in HeLa cells AIP ADVANCES 5, 084803 (2015):
  84. Lee, D., M. M. Rahman, Y. Zhou, S. Ryu. 2015 Confocal Microscopy Indentation Method for Hydrogel. Langmuir. 31(35), 9684-93.
  85. Petro, T. M., Agarkova, I., Zhou, Y., Yolken, R. H., Van Etten, J. L., Dunigan, D. (2015). Response of Mammalian Macrophages to Challenge with the Chlorovirus Acanthocystis turfacea Chlorella Virus 1. J. Virol, 89(23), 12096-107.
  86. Bohovych I, Kastora S, Christianson S, Topil D, Kim H, Fangman T, Zhou Y, Barrientos A, Lee J, Brown AJ, Khalimonchuk O. 2016. Oma1 links mitochondrial protein quality control and TOR signaling to modulate physiological plasticity and cellular stress responses. Mol Cell Biol. 2016 Jun 20. pii: MCB.00156-16. (Epub ahead of print; PMID: 27325672)
  87. Destache CJ, Mandal S, Yuan Z, Kang G, Date AA, Lu W, Shibata A, Pham R, Bruck P, Rezich M, Zhou Y, Vivekanandan R, Fletcher CV, Li Q. 2016 Topical Tenofovir Disoproxil Fumarate Nanoparticles Prevent HIV-1 Vaginal Transmission in a Humanized Mouse Model. Antimicrob Agents Chemother. 2016 May 23;60(6):3633-9. doi: 10.1128/AAC.00450-16.
  88. Navarro-Yepes, J., Anandhan, A., Bradley, E., Bohovych, I., Yarabe, B., de Jong, A., Ovaa, H., Zhou, Y., Khalimonchuk, O., Quintanilla-Vega, B., Franco Cruz, R. (2015). Inhibition of Protein Ubiquitination by Paraquat and 1-Methyl-4-Phenylpyridinium Impairs Ubiquitin-Dependent Protein Degradation Pathways. Mol Neurol. 2016 Oct;53(8):5229-51
  89. Walter ME, Ortiz A, Sondgeroth C, Sindt NM, Duszenko N, Catlett JL, Zhou Y, Valloppilly S, Anderson C, Fernando S, Buan NR. 2016. High-throughput mutation, selection, and phenotype screening of mutant methanogenic archaea. J Microbiol Methods. 2016 Oct 19. pii: S0167-7012(16)30297-4. doi: 10.1016/j.mimet.2016.10.010. 
  90. Gao Y., J. Song, S. Li, C. Elowsky, Y. Zhou, S. Ducharme, Y.M. Chen, Q. Zhou, L. Tan 2016. Hydrogel microphones for stealthy underwater listening. Nat Commun. 2016 Aug 24;7:12316.
  91. Mandal, S., Belshan, M., Holec, A., Zhou, Y., Destache, C. J. (2016). An Enhanced Emtricitabine-Loaded Long-Acting Nanoformulation for Prevention or Treatment of HIV Infection. Antimicrobial agents and chemotherapy. 2016 Dec 27; 61(1) pii: e01475-1. 
  92. Mandal S, Prathipati PK, Kang G, Zhou Y, Yuan Z, Fan W, Li Q, Destache CJ., 2017 Tenofovir alafenamide and elvitegravir loaded nanoparticles for long-acting prevention of HIV-1 vaginal transmission. AIDS. 2017 Feb 20;31(4):469-476. PMID: 28121666 
  93. Tso FY, Sawyer A, Kwon EH, Mudenda V, Langford D, Zhou Y, West J, Wood C., 2017 Kaposi's Sarcoma-Associated Herpesvirus Infection of Neurons in HIV-Positive Patients. J Infect Dis. 2017 Jun 15;215(12):1898-1907. PMID: 27932611
  94. Krishnan B, Massilamany C, Basavalingappa RH, Gangaplara A, Rajasekaran RA, Afzal MZ, Khalilzad-Sharghi V, Zhou Y, Riethoven JJ, Nandi SS, Mishra PK, Sobel RA, Strande JL, Steffen D, Reddy J., 2018 Epitope Mapping of SERCA2a Identifies an Antigenic Determinant That Induces Mainly Atrial Myocarditis in A/J Mice. J. Immunol. 2018 Jan 15; 200(2):523-537. 
  95. Gulati, P. Zhou Y. Elowsky C., Rose, DJ. 2018. Microstructural changes to proso millet protein bodies upon cooking and digestion. J. Cereal Sci. 80, March 2018, Pages 80-86.
  96. Mandal S, Kang G, Prathipati PK, Zhou Y, Fan W, Li Q, Destache CJ. 2019 Nanoencapsulation introduces long-acting phenomenon to tenofovir alafenamide and emtricitabine drug combination: A comparative pre-exposure prophylaxis efficacy study against HIV-1 vaginal transmission. J Control Release. 2019 Jan 28;294:216-225.
  97. Chen, X., Zhou Y, Yu J, 2019 Exosome-like Nanoparticles from Ginger Rhizomes Inhibited NLRP3 Inflammasome Activation. Mol. Pharmaceutics 2019 (April) 16 (6) 2690-2699
  98. Henrick BM, Chew S, Casaburi G, Brown HK, Frese SA, Zhou Y, Underwood MA, Smilowitz JT. 2019 Colonization by B. infantis EVC001 modulates enteric inflammation in exclusively breastfed infants. Pediatr Res. 2019 Dec;86(6):749-757. doi: 10.1038/s41390-019-0533-2.
  99. Zhang J, Yang X, Zhou Y, Fox H, Xiong H. 2019 Direct contacts of microglia on myelin sheath and Ranvier's node in the corpus callosum in rats. J Biomed Res. 2019 Jun 4;33(3):192-200. PMID: 30403198
  100. Mukadam IZ, Machhi J, Herskovitz J, Hasan M, Oleynikov MD, Blomberg WR, Svechkarev D, Mohs AM, Zhou Y, Dash P, McMillan J, Gorantla S, Garrison J, Gendelman HE, Kevadiya BD. 2020 Rilpivirine-associated aggregation-induced emission enables cell-based nanoparticle tracking. Biomaterials. 2020 Feb;231:119669 
  101. Wang R, Y Zhou, N Kalchayanand, DM Harhay, TL Wheeler. 2020 Effectiveness and Functional Mechanism of a Multicomponent Sanitizer against Biofilms Formed by Escherichia coli O157:H7 and Five Salmonella Serotypes Prevalent in the Meat Industry. J Food Prot. 2020 Apr 1;83(4):568-575. doi: 10.4315/0362-028X. JFP-19-393. PMID: 32221560
  102. Allen, D, Y. Zhou, A. Wilhelm, and P Blum. 2020 Intracellular G‑actin targeting of peripheral sensory neurons by the multifunctional engineered protein C2C confers relief from infammatory pain. Nature Sci Rep. 2020 Jul 30;10(1):12789. PMID: 32732905 PMCID: PMC7393082. doi:10.1038/s41598-020-69612-9.
  103. Chitlapilly Dass S, Bosilevac JM, Weinroth M, Elowsky CG, Zhou Y, Anandappa A, Wang R. 2020 Impact of mixed biofilm formation with environmental microorganisms on E. coli O157:H7 survival against sanitization. NPJ Sci Food. 2020 Oct 14;4:16. doi: 10.1038/s41538-020-00076-x. eCollection 2020. PMID: 33083548
  104. Chen X, Liu B, Li X, An TT, Zhou Y, Li G, Wu-Smart J, Alvarez S, Naldrett MJ, Eudy J, Kubik G, Wilson RA, Kachman SD, Cui J, Yu J. 2021. Identification of anti-inflammatory vesicle-like nanoparticles in honey. J Extracell Vesicles. 2021 Feb;10(4):e12069. doi: 10.1002/jev2.12069. Epub 2021 Feb 12. PMID: 33613874
  105. Wang R, Zhou Y, Kalchayanand N, Harhay DM, Wheeler TL. 2021 Consecutive Treatments with a Multicomponent Sanitizer Inactivate Biofilms Formed by Escherichia coli O157:H7 and Salmonella enterica and Remove Biofilm Matrix. J Food Prot. 2021 Mar 1;84(3):408-417. doi: 10.4315/JFP-20-321. PMID: 33108462
  106. H. Roth, F. Bhinderwala, R. Franco, Y. Zhou, and R. Powers* (2021) "DNAJA1 dysregulates metabolism promoting an anti-apoptotic phenotype in pancreatic ductal adenocarcinoma", Journal of Proteome Research, 20(8):3925-3939; July 2021 
  107. Abdelmoaty MM, Yeapuri P, Machhi J, Olson KE, Shahjin F, Kumar V, Zhou Y, Liang J, Pandey K, Acharya A, Byrareddy SN, Mosley RL, Gendelman HE 2021 Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions. Front Immunol 2021 Oct 4;12:741502. doi: 10.3389/fimmu. 2021.741502
  108. Liu B, Li X, Yu H, Shi X, Zhou Y, Alvarez S, Naldrett MJ, Kachman SD, Ro SH, Sun X, Chung S, Jing L, Yu J. 2021. Therapeutic potential of garlic chive-derived vesicle-like nanoparticles in NLRP3 inflammasome-mediated inflammatory diseases. Theranostics. 2021 Sep 7;11(19):9311-9330. doi: 10.7150/thno.60265. eCollection 2021. PMID: 34646372 
  109. Machhi, J. P. Yeapuri, M. Markovic, M. Patel, W. Yan, Y. Lu, J.D. Cohen, M. Hasan, M. Abdelmoaty, Y. Zhou, H. Xiong, X. Wang, R. L. Mosley, H.E. Gendelman, and B.D. Kevadiya 2022. Europium-Doped Cerium Oxide Nanoparticles for Microglial Amyloid Beta Clearance and Homeostasis. ACS Chem. Neurosci. 2022 Apr 20;13(8):1232-1244. PMID: 35312284

Book Chapter

You Zhou (2014). Confocal imaging of nerve cells; Chapter 18, in “Current Laboratory Methods in Neuroscience Research” (H. Xiong and H. Gendelman, Editors). Springer Science + Business Media, New York. 2014, page: 235-247

Karl J. Reinhard, Julia Russ, Isabel Teixeira-Santos, You Zhou, Vaughn M. Bryant (2021). Analysis of Mummy Digestive Tract Contents with Examples of Relevance to Diet, Health, and Hospice. In “The Handbook of Mummy Studies” D. H. Shin, R. Bianucci (eds.), © Springer Nature Singapore Pte Ltd. 2021 April 16 pp1-35

GenBank Publication

The sequence of a polymorphic form of mouse glucocorticoid receptor, which is associated with altered stress response and increased anxiety-type behavior in the SH/SL mouse lines we developed in University of Nebraska-Lincoln, was published on October 15, 2006, at the NCBI GenBank. Accession #: DQ504162.



Use of imaging tools for studies on cell biology, bacteriology, neurosciences, parasitology, pathology, virology

Research and services focuses

  • Cell biology/histology/neurobiology/virology
  • Advanced fluorescence/confocal/electron microscopy and image analysis
  • Immunolabeling fluorescence or electron microscopy
  • Negative staining (viruses, bacteria, exosomes, nanoparticles, and nanofibers) transmission electron microscopy (TEM) for education and research
  • Ultrastructural TEM and topographic scanning electron microscopy (SEM) analyses of tissues, cells, nanoscale particles/fibers.

Participated courses (lecture and lab)

  1. Life 491/891 “Introduction to Core Facilities of Center for Biotechnology” -spring semesters
  2. VBMS-996/998 for a) Transmission electron microscopy (TEM) for veterinary histology/pathology (internship), b) Scanning electron microscopy (SEM) for entomology, forensic science, earth/geoscience, and biological samples
  3. Laboratory courses for negative staining TEM for analysis of bacteriophages/viral particles for undergraduate student from a) Doane University (Bio 110 Inquiry Laboratory: Introduction to Biological Investigation), b) Nebraska Wesleyan University (BIO 190, Gen. Biol.: Inquiry), and c) UNL (Bios 136, Discovery Research: Virus Hunting)—spring and fall semesters.




20% Research, 10% Teaching, 70% Service

Research Areas

  • Bacteriology
  • Molecular and Cellular Biology
  • Parasitology
  • Pathology
  • Virology

Professional Interests

  • Advanced fluorescence/confocal/electron microscopy and live-cell imaging analysis at micro/nano scales.
  • Human and animal cell biology/histology/neurobiology.
  • Immunolabeling fluorescence or electron microscopy.
  • Negative staining (viruses, bacteria, exosomes, nanoparticles, and nanofibers) TEM.
  • Ultrastructural TEM and topographic SEM analyses of tissues, cells, nanoscale particles/fibers.

Professional Society Memberships

  • University of Nebraska Graduate Faculty
  • Nebraska Center for Virology (NCV)
  • Nebraska Center for Redox Biology (RBC)
  • Nebraska Center for Integrative Biomolecular Communication (CIBC)
  • UNMC MSIA Graduate Program
  • University Nebraska NSRI Fellow