Accession Fee $16.00
One to 240 calves $4.50
More than 240 calves $3.75
Retesting of previous positive calves
(must indicate previous case number)
No Charge


View, fill in and print our BVD skin biopsy submission form two pages



(If samples are submitted with no referring veterinarian, we prefer for payment to be submitted with submission. Payment may be made by cash, check or money order only.)

Skin biopsies from any location on the body are sufficient. The most convenient method is to collect an ear notch. A triangle-shaped notch ranging between ¼ and ½ inch on a side is an adequate size. Biopsies should be placed in individual (preferably red-topped) tubes (do NOT use Whirl-Paks) with neutral-buffered formalin. (Additional charges may be assessed if the Diagnostic Center has to place the ear notches in formalin.) Skin samples should not be held in formalin for more than one week prior to submission to the Diagnostic Center. Extended storage in formalin will result in falsely negative tests. The tubes need to be labeled to identify the calf from which the biopsy was collected. Please be sure to fill out the accession form completely, including tube # and animal ID for each ear notch submitted. . (Additional charges may be assessed if the paperwork is not filled out properly.) Please allow 5 - 7 working days for faxing of results.

We can mail additional forms, or a form is available by clicking on the link above this information.

The veterinarian and owner information can be entered on the computer and then the form printed for entering calf identification information by hand.

You may also submit large cases with an Excel file by e-mailing the spread sheet to with the animal ID's in the correct order. Please note on the submission form that you send with the sample, that you are submitting the animal ID's by e-mail. Please fill out the rest of the submission form including the starting and ending tube numbers.

If you have questions, please do not hesitate to contact the Diagnostic Center.

Neutral-Buffered Formalin (pH 7.0)

Formaldehyde, full strength (37-40% formaldehyde) 100 ml
Sodium phosphate dibasic (anhydrous) (Na2HPO4) 6.5 gr
Sodium phosphate monobasic (NaH2PO4) 4.0 gr
Distilled water 900 ml

You may also use 1 part formalin and 9 parts water.

Mailing Address for Correspondence:
Veterinary Diagnostic Center
University of Nebraska - Lincoln
P.O. Box 82646
Lincoln, NE 68583-2646

Telephone: (402) 472-1434
Fax: (402) 472-3094
E mail:

Delivery Address for Sample/Packages and for FedEx/UPS:
Veterinary Diagnostic Center
University of Nebraska - Lincoln
Room 230A NVDC
4040 East Campus Loop North
Lincoln, NE 68583-0907